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HYPHY Package >> Feature Additions >> [ADDITION]Recombination: Single Breakpoint
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Message started by Sergei on Feb 2nd, 2005 at 11:40am

Title: [ADDITION]Recombination: Single Breakpoint
Post by Sergei on Feb 2nd, 2005 at 11:40am
As per Simon's request I have added a simple standard batch file (available with builds after Feb 1st) to look for a single recombination breakpoint.

The algorithm is simple:

Using NJ (with TN93) reconstructed trees we


  • Fit a single tree to the whole alignment, using a user selected model
  • Try to break the alignment at every variable site and fit two separately inferred trees (again, NJ) using the same model (with global parameters shared, but branch lengths inferred independently).
  • Measure goodness of fit by AIC


If the analysis finds a putative breakpoint, more rigorous tests (e.g. KH test - a standard analysis) can be used to assess the significance of phylogenetic incongruence.

This analysis can compensate for rate variation (using an appropriate substitution model) and use codon models for cDNA.

Title: Re: [ADDITION]Recombination: Single Breakpoint
Post by Simon on Feb 11th, 2005 at 9:30am
Dear Sergei,

Seems to work well. A few suggestions:

1. Rather than fit a model as part of the analysis, could a previously fitted model be loaded instead? Perhaps the recombination analysis could also be added to the result processor module.
2. More output would be nice: how about a table with the breakpoints, the AIC, the delta AIC, with optionally, the trees for each partition, a KH test for the significance of the difference in trees, and some tree distance measures. It might also be good to tally the sites that are contributing to the conflicting phylogenetic signal, but I'd have to think a bit how best to present these.

Best,
Simon

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