HyPhy message board
http://www.hyphy.org/cgi-bin/hyphy_forums/YaBB.pl
Datamonkey Server >> Datamonkey bugs >> Must all coding regions be complete in an alignment?
http://www.hyphy.org/cgi-bin/hyphy_forums/YaBB.pl?num=1393431661

Message started by Alan on Feb 26th, 2014 at 8:21am

Title: Must all coding regions be complete in an alignment?
Post by Alan on Feb 26th, 2014 at 8:21am
I wish to analyze an alignment of two coding regions from a single gene.  When I upload the NEXUS file, specifying "Codon" as the data type, Datamonkey is telling me that it is finding stop codons all over the alignment (in every OTU).  However when I look at all three reading frames in Sequencher, it is clear that Frame 3 is devoid of stop codons, and I can see towards the end of the second exon sequence the characteristic amino acid sequence that earmarks this gene (WRKY).  I cannot for the life of me figure out why Datamonkey is mis-reading this file, unless it is because the 5' coding region is incomplete.  Could that be the problem?

HyPhy message board » Powered by YaBB 2.5.2!
YaBB Forum Software © 2000-2024. All Rights Reserved.