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Methodology Questions >> How to >> Positive Selection on non-coding dataset http://www.hyphy.org/cgi-bin/hyphy_forums/YaBB.pl?num=1400364063 Message started by Aisha_Pisces on May 17th, 2014 at 3:01pm |
Title: Positive Selection on non-coding dataset Post by Aisha_Pisces on May 17th, 2014 at 3:01pm
Hi,
I am interested to detect signatures of postive selection on non-coding dataset. For that, i subjected my dataset to HyPhy 'noncodingselection.bf' using intronic sequences as neutral proxy. However, i don't seem to interpret the output. Here is the output: number of replicates for likelihood fitting= 10 Intron(s) used as neutral proxy with HKY85 model. Branch specific test with foreground branch Human NULL= Class 1: Negative selection in FG and BG. Class 2: Neutral evolution in FG and BG. ALTERNATE= Class 1: Negative selection in FG and BG. Class 2: Neutral evolution in FG and BG. Class 3: Negative selection in BG, Positive in FG. Class 4: Neutral evolution in BG, Positive in FG. *** Null model *** inverse kappa: 0.00589578813346601 f0: 0.2426574443737675 f1: 0.02096646396237873 f2: 0.7363760916638536 zeta0: 0.03093970353822078 zeta1: 1 *** Alternate model *** inverse kappa: 0.01619338026668704 f0: 0.2427677498110386 f1: 0.02096177132117792 f2: 0.7362704788677835 f3: 0.05852031028723284 zeta0: 0.0309385053561257 zeta1: 1 zeta2: 0.005907867454639304 Lk null = -9831.975230914895 Lk alt = -9831.973668884511 LRT = 0.003124060767731862 LRT p-value = 0.9554268099 Moreover, the program asked for either to apply BEB and NEB. I applied BEB and got a table as a result, having three columns named Negative, neutral and positive selection. But i am at a loss to understand the values in those columns.. Is there any one who can simplify all this for me?? In dire need of a positive response. Thanks! |
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