Welcome, Guest. Please Login
YaBB - Yet another Bulletin Board
 
  HomeHelpSearchLogin  
 
KH Testing (Read 1781 times)
Rachael
YaBB Newbies
*
Offline


Feed your monkey!

Posts: 2
KH Testing
Feb 15th, 2010 at 8:13am
 
Hello,

I have a couple questions about using the KH test with GARD:

1)  In your paper, you use the SH to process your GARD results, but not in the single breakpoint analysis.  Do you suggest using the KH test to test phylogenetic congruence for the single breakpoint analysis as well?

2)  If you do suggest this, what is the best way to implement it?  I have run the batchfile KHTest.bf using the trees and breakpoint found when using SingleBreakpointRecomb.bf.  I get a p-value for 1st and 2nd partitions but am not sure how to interpret this result.  Does a significant p-value for either partition indicate recombination?  Or do both partitions have to have a significant p-value?

3)  In using the KH test to process GARD results as well as single breakpoint analysis results, is some correction for multiple testing implemented?

Thank you for your help!

Rachael
Back to top
 
 
IP Logged
 
Sergei
YaBB Administrator
*****
Offline


Datamonkeys are forever...

Posts: 1658
UCSD
Gender: male
Re: KH Testing
Reply #1 - Feb 15th, 2010 at 8:43am
 
Hi Rachael,

To answer your questions

1). For the set of two trees (left and right of a breakpoint), KH and SH tests are equivalent.
2). GARD as implemented on Datamonkey.org already includes KH testing. Within HyPhy, GARDProcessor.bf (a standard analysis under 'Recombination') will also run KH testing. KHTest.bf will preform two tests: does tree 1 fit partition 1 better than tree 2, and does tree 2 fit partition 2 better than tree 1. If the maximum of these p-values is small (e.g. <0.05 or <0.025 etc), you can conclude that the topologies are discordant.
3). GARDProcessor.bf and datamonkey.org implement a simple Bonferroni MT correction.

Cheers,
Sergei
Back to top
 

Associate Professor
Division of Infectious Diseases
Division of Biomedical Informatics
School of Medicine
University of California San Diego
WWW WWW  
IP Logged