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Ancestral state reconstructions in HyPhy (Read 2845 times)
Dan Fulop
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Ancestral state reconstructions in HyPhy
Jul 26th, 2011 at 2:29pm
 
Hi,

I'm wondering if the ancestral state reconstruction methods available in Datamonkey under the ASR module are also implemented/available in HyPhy.

Obviously, they're implemented in HyPhy as Datamonkey uses HyPhy on the backend, etc.  But, I can't find any such analyses under the "Standard Anlyses" menu in the HyPhy GUI, so I can't see what options I need to code a script, etc.  ...I haven't yet looked in the command line...

Thanks,
Dan.
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Sergei
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Re: Ancestral state reconstructions in HyPhy
Reply #1 - Jul 26th, 2011 at 2:36pm
 
Hi Dan,

They are accessible in a somewhat limited fashion. Take a look at post_ancestors.bf in TemplateBatchFiles (simple case).

Sergei
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Dan Fulop
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Re: Ancestral state reconstructions in HyPhy
Reply #2 - Jul 26th, 2011 at 2:48pm
 
Hi Sergei,

Thanks for the prompt reply.

A simple implementation is actually better than what we're using right now.  Plus, I don't think we could apply the more complex methods to ~14K genes in a reasonable amount of time.

Cheers,
Dan.
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Dan Fulop
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Re: Ancestral state reconstructions in HyPhy
Reply #3 - Jul 26th, 2011 at 3:28pm
 
I can feed it either a nucleotide or a codon model fit, right?

We're looking at losses and gains of stop codons, so using a codon model for the reconstructions would be more appropriate.  Would you agree?
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