Russell
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Curious HyPhy user
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Dear Dr. Sergei I have few question, i want to go for codon selection analysis (SLAC,FEL, REL, FUBAR). I have screened with GARD and SBP for recombination. 1. GARD- shows two breakpoint- 1597 and 2482 position, but SBP show one breakpoint -2495 position for same multiple sequence alignment (MSA). Why this discrepancy in result, which one to take.
2. How to correct for recombination- Methology, a. whether to download GARD Nexus output file after screening for GARD analysis and upload it in, analyze ur data option in datamonkey , to proceed for (SLAC,FEL, REL, FUBAR) analysis ( or ) b. otherwise based on breakpoint position, i have to split all the sequences of alignment, into fragment , then align each fragment section to MSA, upload each fragment section MSA to proceed for analysis in datamonkey server.
3. whether i can use Parris directly on alignment containing recombination, inaddition to above (SLAC,FEL, REL, FUBAR) analysis.
I am novice to datamonkey, selection analysis. Some question may look silly, sorry. Kindly answer my queries. I have also referred this article “Phylogenetics- Robust inference of positive selection from recombining coding sequences- Konrad Scheffler, Darren P. Martin and Cathal Seoighe.
Thank u
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