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Dear Sergei,
It forgives me if this error will be plus an error newbie. I tried to run the new options that you wrote in your post, but the HYPHY returned this error , the log is below:
/HYPHY 0.9920070328beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Basic Analyses (2) Codon Selection Analyses (3) Compartmentalization (4) Data File Tools (5) Miscellaneous (6) Model Comparison (7) Molecular Clock (8) Phylogeny Reconstruction (9) Positive Selection (10) Recombination (11) Selection/Recombination (12) Relative Rate (13) Relative Ratio (14) Substitution Rates
Please select type of analyses you want to list (or press ENTER to process custom batch file):7 ***************** FILES IN 'Molecular Clock' ***************** (1) Test for the presence of a global molecular clock with DATED sequences. Evolutionary rates and dates for internal nodes are estimated as well. This analysis is effectively an extended version of Andrew Rabmaut's TipDate methodogoly. (2) Test for the presence of a global molecular clock on the tree using its root (the resulting clock tree is unrooted, but one of the root branches can be divided in such a way as to enforce the clock). (3) Test for the presence of a local molecular clock. Every subtree of the given tree is subjected to the clock constraint, while the remainder of the tree is free of the clock constraint. (4) Test for the presence of a global molecular clock on the tree. The tree is rooted at every possible branch. (5) Infer dates of ancestral nodes for a sample of DATED sequences using a rate-distribution genetic algorithm to allow for multiple substituion rates. This is a frequentist alternative to the relaxed clock analyses implemented by Drummond and Rambaut in BEAST. [MPI Enabled] (6) Process a .samples file generated with GADatedClock
Please select the file you want to use (or press ENTER to return to the list of analysis types):5
(/root/HYPHY/TemplateBatchFiles/) Locate a nucleotide data file:/root/NSP4orfdatedmafftseqrootedtree.phy +--------------------------+ | Select a standard model. | +--------------------------+ (CUSTOM):Custom 4x4 reversible model (defined as in 010023) with choice of several equilibrium frequencies options. (F81):Felsenstein 81 (F81).Rate heterogeneity (and HM spatial correlation) optional. (F84):Felsenstein 84. Rate heterogeneity (and HM spatial correlation) optional. (F84P):Felsenstein 84, compatible with PHYLIP's dnaml version. User chooses ts/tv ratio. Rate heterogeneity (and HM spatial correlation) optional. (GRM):General Reversible Model.Local or global parameters. Possible Rate heterogeneity (and HM spatial correlation). (HKY85):Hasegawa et al.,1985. Local or global parameters. Possible Rate heterogeneity (and HM spatial correlation). (JC69):Jukes-Cantor 1969.Rate heterogeneity (and HM spatial correlation) optional. (K2P):Kimura 2 parameter (K2P).Local or global parameters. Possible Rate heterogeneity (and HM spatial correlation). (K3ST):Kimura(1981) 3 substitution type model. Local or global parameters. Possible Rate heterogeneity (and HM spatial correlation). (NRM):Non reversible model, constrained to have observed counts as its equilibrium frequencies. 11 parameters (8 rate parameteres + 3 frequency parameters). (NRM+FREQS):Non reversible model, NOT constrained to have observed counts as its equilibrium frequencies. 11 parameters. (TRN):Tamura and Nei 1993. Local or global parameters. Local or global parameters. Possible Rate heterogeneity (and HM spatial correlation).
Please type in the abbreviation for the model you want to use:custom
Please enter a 6 character model designation (e.g:010010 defines HKY85):010010 +-------------+ |Model Options| +-------------+ (1):[Local] All model parameters are estimated independently for each branch. (2):[Global] Model parameters are shared by all branches, branch lengths are estimated independently. (3):[Global w/variation] Model parameters are shared by all branches, branch lengths come from a user-chosen distribution, whose parameters are estimated. (4):[Global w/variation+HM] Model parameters are shared by all branches, branch lengths come from a user-chosen distribution, whose parameters is estimated; rates at adjacent sites are correlated via a simple Hidden Markov model with an autocorrelation parameter lambda.
Please choose an option (or press q to cancel selection):3 +-------------------+ |Choose Distribution| +-------------------+ (1):[Gamma] Gamma distribution with shape parameter alpha. Mean: alpha, variance: 1/alpha. (2):[Beta] Two-parameter beta distrubution. Mean: betaP/(betaQ+betaP), variance: betaP*betaQ/((betaP+betaQ)^2 (betaP+betaQ+1)). (3):[Normal(x>0)] Two-parameter normal distrubution restriced to x>0. Mean parameter: mu, variance parameter: sigma^2. (4):[Log normal] Unit mean log normal distribution with parameter sigma. Variance: 1/[exp(sigma^2)-1]. (5):[Gamma+Inv] Gamma distribution with shape parameter alpha and mean 1/(1-P) and a zero rate class for invariable sites with weight P. (6):[Gamma Quadratures] Gamma distribution with shape parameter alpha. Mean: alpha, variance: 1/alpha. Discretized using Gauss-Laguerre quadratures, matching 2N-1 moments, with N = the number of rate classes. (7):[Inv Class] A mixture of point masses at 0 and 1, with proportions P and (1-P). Mean: (1-P), variance P(1-P). Parameters: P - the proportion of invariable sites. (8):[General Discrete] Unit mean general discrete. (9):[Beta-Gamma] Gamma distribution with shape parameter alpha partitioned by a beta distribution. (10):[Beta-Lognormal] Mean one log normal distribution with parameter sigma, partitioned by the beta. Variance: 1/[exp(sigma^2)-1]. (11):[Normal(x>0) mod Beta] Normal (x>0) distribution with parameter sigma, partitioned by the beta. Mean parameter: mu, variance parameter: sigma^2. (12):[Beta-Uniform] Mean 1 piecewise uniform on [0,1] and [1,R] partitioned by the beta. (13):[Uniform] Mean 1 piecewise uniform on [0,1] and [1,R]. (14):[Beta-(Gamma+Inv)] Mixture of gamma mod beta and an invariant class partitioned by the beta.
Please choose an option (or press q to cancel selection):5
How many rate classes should we have?4 +---------------+ |Rate Parameters| +---------------+ (1):[Estimated] Rate parameters are inferred by maximum likelihood. (2):[User] Rate parameters are fixed at user specified values
Please choose an option (or press q to cancel selection):2 Enter the rate for AC (relative to AG rate):7.10000 +-----------------------+ |Equilibrium Frequencies| +-----------------------+ (1):[Observed] Nucleotide frequencies collected from the data file will be used as equilibrium frequencies. (2):[Equal] Equal (.25) frequencies are used as equilibrium frequencies (3):[Custom] Define your own vector of frequencies.
Please choose an option (or press q to cancel selection):1
A tree was found in the data file: (13Hum1995:4120.2,(107Hum2002:0,((((12Hum2004:0,(11Hum2004:0,(((((((4Hum1999:0,( 123Cap1998:0,239Can1980:7.17531):2.18521):154.789,(((((((((((((((((((((((((((((( ((((2Hum2000:4.91975,118Lam1981:3.16657):1.00259,242Equ1976:0.54289):1.67751,77H um1993:1.81005):1.04422,(((((1Sim1958:1.73424,19Hum2004:1.55462):1.86337,((((((( ((((((((((((((((((((((((((((((((((((((((3hum2000:0,282Hum2002:0.019575):0.057103 6,14Hum2002:0.0425057):0,15Hum2000:0):0.364404,16Hum2001:0.133973):0,180Hum1990: 0):0.00106258,179Hum1990:0):0.00282136,181Hum1990:0):0.0290651,110Hum1998:0):0.0 0500024,274Hum2003:0):0.00299513,169Hum1998:0):0.00439328,155Hum1997:0):0.017498 3,289Hum2001:0):0.0017609,171Hum1998:0):0.00310972,275Hum2003:0):0.00267115,170H um1998:0):0.002041,164Hum1998:0):0.00204142,167Hum1998:0):0.00202064,156Hum1997: 0):0.00191157,272Hum2003:0):0.00433435,264Hum2004:0):0.00100582,166Hum1998:0):2. 00307e-06,168Hum1996:0):0.0262291,(((82Hum1996:0,((((95Hum1996:0,112Hum1999:0.01 62303):0.00529217,279Hum2002:0):0,287Hum2001:0):0,286Hum2002:0):0.00310414):8.12 184e-05,85Hum1997:0):0.004212,91Hum1996:0):0.00762451):0,276Hum2003:0):0.0048001 4,87Hum1996:0):0.0773933,206Hum1978:0):0.0115426,(130Hum1980:0.0296187,(267Hum20 04:0.0317216,268Hum2004:0.016421):0.0167293):0.00215788):0.0109373,202Hum1995:0. 0130884):0.0593484,((209Hum1993:0,((210Hum1994:0,(211Hum1994:0.00190364,212Hum19 95:0):2.085e-06):0.00855432,235Hum1994:0.000207175):0):0.0001328,220Hum1995:0):0 .00676737):0.000622239,233Hum1974:0):0.00309869,((84Hum1996:0.00196616,89Hum1997 :0):0.0103962,(((((((((127Hum1990:0.000341553,(128Hum1991:0.00407388,129Hum1991: 0):0.00586837):0.00183738,139Hum1990:0.011653):0.00335238,((159Hum1997:0.0013182 9,((161Hum1997:0,236Hum1997:0.00403527):0.00406097,237Hum1997:0):0.00298694):0.0 0155052,162Hum1997:0.000307539):0.00928197):0.00205181,(207Hum1994:0,208Hum1994: 0.0040529):0.00592305):0,134Hum1990:0):0.00308755,141Hum1986:0.00372256):0.00240 187,138Hum1989:0.00192639):0.00178473,201Hum1989:0):0.0357136,132Hum1983:0.02533 04):0.00844376):0.00161787):0.000861525,232Hum1996:0.00783089):0.000390435,(135H um1981:0.00388288,(271Hum2003:0.0149456,277Hum2003:0.00107206):0.00886243):0.005 40648):0.000649812,((194Hum1985:0.00199558,196Hum1985:0.00205918):0,195Hum1985:0 ):0.00197891):0.000694978,(((((70Hum1992:0.00379096,73Hum1991:0.00482068):0,71Hu m1992:0):0.00772346,74Hum1991:0.00417174):0,72Hum1992:0):0.000147237,75Hum1990:0 ):0.00183163):0.00643038,((124Hum1995:0,125Hum1995:0.00404394):0.00332144,154Hum 1997:0.00332664):0.00477916):0.000185696,160Hum1997:0):0.0055867,111Hum1993:0):0 .0542895,178Hum1990:0):0,(136Hum1977:0,203Hum1993:0.0756656):0.0709806):0.158305 ,176Hum1980:0):0,((((131Hum1981:0,((((((133Hum1982:0.0942778,254Hum1975:0.093833 1):0.0624907,231Hum1998:0):0.0510279,(((((182Hum1988:0,(183Hum1988:0,184Hum1988: 0.00394963):0.00657816):0.00113508,185Hum1988:0):0.0196337,204Hum1992:0):0.03968 19,255Hum1982:0):0,205Hum1981:0):0.166457):0.0709423,261Hum1988:0):0.092376,142H um1992:0.30933):0,260Hum1990:0):0.0121973):0.0568728,262Hum1987:0):0,263Hum1986: 0):0.0536069,253Hum1981:0):0.0187805):0.105537,(17Hum1997:0.129449,177Hum1984:0) :0.0834963):0.465497,((((5Hum2003:0,6Hum2001:0.207129):0.511905,(7Hum2000:0,53Po r2004:0.215376):0.923543):0.832293,(109Hum1997:0.251246,175Hum1986:0):0.401185): 0,68Hum1995:0):0.93743):1.47251,(((((9Bov2005:0.204165,51Por2004:0):0.0811759,11 4Hum1990:0):0.859461,238Por1987:0.58979):1.45801,(106Por1980:0.227754,(243Equ197 5:0.0747096,((244Por1987:0.0842832,246Por1987:0.122436):0.134412,252Por1976:0):0 .104454):0.134456):2.22517):0.255884,21Hum1997:2.65024):0.258209):0.84812,((((11 3Hum2000:0.0151591,(((122Rab1996:0.119551,290Lap2004:0):0.0764512,248Lap1988:0.0 203089):0,251Rot1984:0):0.0704559):0.063765,247Lap1988:0):2.71223,(119Rab2001:0. 17806,120Rab2001:0):2.76861):0.0773764,121Rab2001:2.74894):0.9595):0.2607,(240Eq u1981:2.48731,241Equ1981:2.47287):1.14142):0.155344):0.768818,249Lap1978:2.66):0 .682119,(50Sim1990:0.0626322,172Bov1981:0):2.31971):0.672659,117Bov2001:1.36566) :0.889501):1.40815,98Hum1996:0):0.241922,108Hum1998:0):0,140Hum1992:0):0.990655, 63Hum1997:0):0.0114751,257Hum1993:0):0,42Hum1997:0):0.361657,151Hum1996:0):0,67H um1995:0):0.302637,20Hum2004:5.50543):0.943886,65Hum1996:0):0.00945965,283Hum200 2:0):0.147208,187Hum1994:0):0.022128,100Hum1996:0):0.00523764,69Hum1996:0):0.014 3003,105hum1996:0):0,234Hum1996:0):0,78Hum1990:1.54561):0.615906,278Hum2002:0):0 .317713,8Hum1997:0):0.326008,64Hum1996:0):0,258Hum1981:0):0.234658,24Hum1997:0): 0.245123,256Hum1993:0):0,((((((79Hum1990:0,(153Hum1997:0,(291Mur1982:2.35224,292 Mur1981:0.271833):241.812):39.9998):12.3761,173Bov1973:0):10.9326,(((101Hum1996: 0,((137Hum1980:7.62351,245Por1987:0):3.90022,250Lap1983:0):3.70695):0,152Hum1997 :0):1.1622,186Hum1976:0):44.2382):0,193Hum1981:0):0.99991,189Hum1993:0):1.29879, 192Hum1982:0):2.39156):9.45341,190Hum1992:0):0.991022,191Hum1983:0):5.42061,62Hu m1982:0):0,43Hum1997:0):0,144Lap1978:0):23.3456,56Fel1993:0):0):222.876,55Fel199 3:0):1.31013,57Fel1993:0):3.86669,61Fel1989:0):0,(116Hum2000:466.916,126Hum1992: 120.895):732.074):564.737,58Fel1993:0):672.1):0):0,174Bov1973:0):3238.72,145Hum1 993:3858.34):10540.1,115Hum1998:0):0))
Would you like to use it:(Y/N)?Y
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